A Practical Guide to the Study of Calcium in Living Cells

Nonfiction, Science & Nature, Science, Biological Sciences, Cytology, Health & Well Being, Medical, Specialties, Internal Medicine, Neuroscience
Cover of the book A Practical Guide to the Study of Calcium in Living Cells by Leslie Wilson, Paul T. Matsudaira, Richard Nuccitelli, Elsevier Science
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Author: Leslie Wilson, Paul T. Matsudaira, Richard Nuccitelli ISBN: 9780080859385
Publisher: Elsevier Science Publication: April 25, 1994
Imprint: Academic Press Language: English
Author: Leslie Wilson, Paul T. Matsudaira, Richard Nuccitelli
ISBN: 9780080859385
Publisher: Elsevier Science
Publication: April 25, 1994
Imprint: Academic Press
Language: English

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

  • Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
  • Fluorescent dyes
  • Luminescent proteins
  • Includes techniques for perturbing intracellular Ca2+
  • Covers detailed methodology plus problems and pitfalls of each technique
  • Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
  • Color plates illustrate techniques such as
  • Confocal ratio-imaging
  • Use of aequorin
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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

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