Author: | Paul Verkade, Thomas Muller-Reichert | ISBN: | 9780123914385 |
Publisher: | Elsevier Science | Publication: | October 23, 2012 |
Imprint: | Academic Press | Language: | English |
Author: | Paul Verkade, Thomas Muller-Reichert |
ISBN: | 9780123914385 |
Publisher: | Elsevier Science |
Publication: | October 23, 2012 |
Imprint: | Academic Press |
Language: | English |
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.