Microarray Technology in Practice

Nonfiction, Science & Nature, Science, Biological Sciences, Biotechnology, Genetics, Technology
Cover of the book Microarray Technology in Practice by Lisa A. Meadows, Roslin R. Russell, Steve Russell, Elsevier Science
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Author: Lisa A. Meadows, Roslin R. Russell, Steve Russell ISBN: 9780080919768
Publisher: Elsevier Science Publication: November 21, 2008
Imprint: Academic Press Language: English
Author: Lisa A. Meadows, Roslin R. Russell, Steve Russell
ISBN: 9780080919768
Publisher: Elsevier Science
Publication: November 21, 2008
Imprint: Academic Press
Language: English

Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique.

  • Cohesive overview of the technology and available platforms, followed by detailed discussion of experimental design and analysis of microarray experiments
  • Up-to-date description of normalization methods and current methods for sample amplification and labeling
  • Deep focus on oligonucleotide design, printing, labeling and hybridization, data acquisition, normalization, and meta-analysis
  • Additional uses of microarray technology such as ChIP (chromatin immunoprecipitation) with hybridization to DNA arrays, microarray-based comparative genomic hybridization (CGH), and cell and tissue arrays
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Using chips composed of thousands of spots, each with the capability of holding DNA molecules corresponding to a given gene, DNA microarray technology has enabled researchers to measure simultaneously gene expression across the genome. As with other large-scale genomics approaches, microarray technologies are broadly applicable across disciplines of life and biomedical sciences, but remain daunting to many researchers. This guide is designed to demystify the technology and inform more biologists about this critically important experimental technique.

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