Optical Fluorescence Microscopy

From the Spectral to the Nano Dimension

Nonfiction, Science & Nature, Science, Other Sciences, Molecular Biology, Biological Sciences
Cover of the book Optical Fluorescence Microscopy by , Springer Berlin Heidelberg
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Author: ISBN: 9783642151750
Publisher: Springer Berlin Heidelberg Publication: November 25, 2010
Imprint: Springer Language: English
Author:
ISBN: 9783642151750
Publisher: Springer Berlin Heidelberg
Publication: November 25, 2010
Imprint: Springer
Language: English

In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

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In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.

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