Quantification of Circulating Proteins

Theory and applications based on analysis of plasma protein levels

Nonfiction, Health & Well Being, Medical, Specialties, Internal Medicine, Hematology
Cover of the book Quantification of Circulating Proteins by Wim Th. Hermens, George M. Willems, Marja P. Visser, Springer Netherlands
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Wim Th. Hermens, George M. Willems, Marja P. Visser ISBN: 9789400976603
Publisher: Springer Netherlands Publication: December 6, 2012
Imprint: Springer Language: English
Author: Wim Th. Hermens, George M. Willems, Marja P. Visser
ISBN: 9789400976603
Publisher: Springer Netherlands
Publication: December 6, 2012
Imprint: Springer
Language: English

Less than 50 years ago it was discovered that steady-state protein concentrations in plasma are the net result of continuous elimination and synthesis of protein molecules. The first quanti­ tative studies on the turnover and distribution of plasma pro­ teins were made around 1950, after the introduction of radio­ labeled protein preparations. Around 1970, another development in quantitative interpre­ tation of circulating proteins was initiated in clinical enzy­ mology. Estimation of cumulative release into plasma of cellular enzymes can be helpful in a variety of diseases to assess the extent of tissue damage and to evaluate therapy. Enzymes can be considered as biological tracers, i.e. minute quantities of protein can be accurately determined by their spe­ cific catalytic activities. However, radioactive tracers permit direct estimates of turnover and distr ibution by measurement of excreted radioactivity, possibilities that are not available for enzymes. Consequently, only a few techniques used in tracer studies with radiolabeled proteins can be applied to circulating tissue enzymes and this may explain the lack of communication between the fields of plasma protein metabolism and quantitative clinical enzymology. In the present study a summary is given of the basic methods used in both fields, with emphasis on the equivalence of various models and formalisms used by different authors. It is shown that major limitations in the study of circulating tissue enzymes can be overcome if two different, but simultaneously released, en­ zymes can be measured. The resulting method will also be applied to plasma protein metabolism.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Less than 50 years ago it was discovered that steady-state protein concentrations in plasma are the net result of continuous elimination and synthesis of protein molecules. The first quanti­ tative studies on the turnover and distribution of plasma pro­ teins were made around 1950, after the introduction of radio­ labeled protein preparations. Around 1970, another development in quantitative interpre­ tation of circulating proteins was initiated in clinical enzy­ mology. Estimation of cumulative release into plasma of cellular enzymes can be helpful in a variety of diseases to assess the extent of tissue damage and to evaluate therapy. Enzymes can be considered as biological tracers, i.e. minute quantities of protein can be accurately determined by their spe­ cific catalytic activities. However, radioactive tracers permit direct estimates of turnover and distr ibution by measurement of excreted radioactivity, possibilities that are not available for enzymes. Consequently, only a few techniques used in tracer studies with radiolabeled proteins can be applied to circulating tissue enzymes and this may explain the lack of communication between the fields of plasma protein metabolism and quantitative clinical enzymology. In the present study a summary is given of the basic methods used in both fields, with emphasis on the equivalence of various models and formalisms used by different authors. It is shown that major limitations in the study of circulating tissue enzymes can be overcome if two different, but simultaneously released, en­ zymes can be measured. The resulting method will also be applied to plasma protein metabolism.

More books from Springer Netherlands

Cover of the book Organ Allocation by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Scientific Uncertainty and Its Influence on the Public Communication Process by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Best Practices in Marketing and their Impact on Quality of Life by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Developments in Dairy Chemistry—3 by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Approaches to Legal Rationality by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Methane Production from Agricultural and Domestic Wastes by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Red Clover Science by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book The Ethics of Business in a Global Economy by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Introduction to Marine Genomics by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Life the Human Being between Life and Death by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Commonsense Cardiology by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Introduction to Trade Mark Law in the Benelux by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Public Policy Making in the Western Balkans by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Regional Environmental Changes in Siberia and Their Global Consequences by Wim Th. Hermens, George M. Willems, Marja P. Visser
Cover of the book Electronic Engineering and Computing Technology by Wim Th. Hermens, George M. Willems, Marja P. Visser
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy