Transgenic Models in Endocrinology

Nonfiction, Health & Well Being, Medical, Specialties, Internal Medicine, Endocrinology & Metabolism, General
Cover of the book Transgenic Models in Endocrinology by Maria G. Castro, Springer US
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Maria G. Castro ISBN: 9781461516330
Publisher: Springer US Publication: December 6, 2012
Imprint: Springer Language: English
Author: Maria G. Castro
ISBN: 9781461516330
Publisher: Springer US
Publication: December 6, 2012
Imprint: Springer
Language: English

The dramatic recent expansion in genomic information has motivated the development of new approaches to characterize gene expression and function. A critical issue for both basic and clinical endocrinologists is the physiological role of genes involved in regulating endocrine functions. Transgenic technologies allow the translation of genotypic information into specific phenotypes by using gene overexpression or loss of specific gene functions. Murine functional genomics is thus of central importance in modem biomedical endocrine research. Although mice are at present, the preferred mammalian species for genetic manipulations because of the availability of pluripotent embryonic. stem cells and inbred strains and the relatively low breeding and maintenance costs, transgenic rats have also been generated and used to study endocrine physiology. The two basic techniques used in the creation of transgenic animal models are integration of foreign DNA into a fertilized oocyte by random chromosomal insertion and homologous recombination in embryonic stem cells that are then introduced into zygotes. Transgenic mice and rats serve as sophisticted tools to probe protein function, as models of human disease, and as hosts for the testing of gene replacement and other therapies. Embryonic stem cell libraries for mouse gene deletion are being developed, which will make it possible to generate knockout mice rapidly and without the need to analyze gene structure, construct targeting vectors, and screen embryonic stem cell clones. A novel approach to transgenesis for the expression of DNA within adult differentiated neuroendocrine cells in vivo is using viral vectors.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

The dramatic recent expansion in genomic information has motivated the development of new approaches to characterize gene expression and function. A critical issue for both basic and clinical endocrinologists is the physiological role of genes involved in regulating endocrine functions. Transgenic technologies allow the translation of genotypic information into specific phenotypes by using gene overexpression or loss of specific gene functions. Murine functional genomics is thus of central importance in modem biomedical endocrine research. Although mice are at present, the preferred mammalian species for genetic manipulations because of the availability of pluripotent embryonic. stem cells and inbred strains and the relatively low breeding and maintenance costs, transgenic rats have also been generated and used to study endocrine physiology. The two basic techniques used in the creation of transgenic animal models are integration of foreign DNA into a fertilized oocyte by random chromosomal insertion and homologous recombination in embryonic stem cells that are then introduced into zygotes. Transgenic mice and rats serve as sophisticted tools to probe protein function, as models of human disease, and as hosts for the testing of gene replacement and other therapies. Embryonic stem cell libraries for mouse gene deletion are being developed, which will make it possible to generate knockout mice rapidly and without the need to analyze gene structure, construct targeting vectors, and screen embryonic stem cell clones. A novel approach to transgenesis for the expression of DNA within adult differentiated neuroendocrine cells in vivo is using viral vectors.

More books from Springer US

Cover of the book Theoretical Perspectives in Environment-Behavior Research by Maria G. Castro
Cover of the book Copolymerization by Maria G. Castro
Cover of the book Contract Analysis and Design for Supply Chains with Stochastic Demand by Maria G. Castro
Cover of the book Ultrasound in Medicine by Maria G. Castro
Cover of the book Coping with Physical Illness by Maria G. Castro
Cover of the book Identifying, Assessing, and Treating Self-Injury at School by Maria G. Castro
Cover of the book The Heart in Diabetes by Maria G. Castro
Cover of the book Advances in Corrosion Science and Technology by Maria G. Castro
Cover of the book The Biosynthesis of Polysaccharides by Maria G. Castro
Cover of the book Infection Control by Maria G. Castro
Cover of the book Contemporary Masters in Criminology by Maria G. Castro
Cover of the book Handbook of Nonverbal Assessment by Maria G. Castro
Cover of the book Cross-Linguistic Study of Acquired Reading Disorders by Maria G. Castro
Cover of the book The Social and Economic Impact of New Technology 1978–84: A Select Bibliography by Maria G. Castro
Cover of the book Before it’s Too Late by Maria G. Castro
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy