Analysis of Rhm51, a DNA Recombinational Repair Gene in the Rice Blast Fungus

Nonfiction, Health & Well Being, Medical, Medical Science, Microbiology
Cover of the book Analysis of Rhm51, a DNA Recombinational Repair Gene in the Rice Blast Fungus by Sali Atanga Ndindeng, GRIN Verlag
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Sali Atanga Ndindeng ISBN: 9783656090007
Publisher: GRIN Verlag Publication: December 27, 2011
Imprint: GRIN Verlag Language: English
Author: Sali Atanga Ndindeng
ISBN: 9783656090007
Publisher: GRIN Verlag
Publication: December 27, 2011
Imprint: GRIN Verlag
Language: English

Doctoral Thesis / Dissertation from the year 2010 in the subject Biology - Micro- and Molecular Biology, grade: A, , course: Molecular Plant-microbe interaction - Agriculture/Rice Blast Disease/pathogenic shift/DNA double strand break repair, language: English, abstract: The ortholog of the Saccharomyces cerevisiae recombinational repair gene RAD51, Rhm51 was cloned from the genome of rice blast fungus Magnaporthe oryzae in order to clarify its role in the variability and pathogenicity. Rhm51 is a single copy gene, and is constitutively expressed at low levels during the cell cycle and at higher levels when the pathogen is grown in media supplemented with DNA damaging agents. We disrupted Rhm51 in two different strains of M. oryzae and both mutants showed reduced growth with conidia and colonies being highly sensitive to DNA double strand break-inducing agents. In addition, they lost the ability to undergo homologous recombination in adeA gene targeting. Mutants showed reduction in conidiation compared to the wild-type strains. Although mutants infected compatible rice cultivars, they produced significantly smaller and fewer lesions compared to wild-type strains and this might be due to the reduction in the amount of appressoria formed. DNA double-strand breaks (DSBs) quantified by the comet assay showed that loss of Rhm51 resulted in the accumulation of DSBs in mutant. Microscopic observation of nuclei and reactive oxygen species (ROS) production during hyphal growth and following appressoria formation revealed impaired mitotic entry, autophagic cell death and ROS production in Rhm51 deletion mutants. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.These results for the first time show that Rhm51 is involved in growth and pathogenicity of M. oryzae through the accurate repair of DSBs that may arise during vegetative and infective growth.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Doctoral Thesis / Dissertation from the year 2010 in the subject Biology - Micro- and Molecular Biology, grade: A, , course: Molecular Plant-microbe interaction - Agriculture/Rice Blast Disease/pathogenic shift/DNA double strand break repair, language: English, abstract: The ortholog of the Saccharomyces cerevisiae recombinational repair gene RAD51, Rhm51 was cloned from the genome of rice blast fungus Magnaporthe oryzae in order to clarify its role in the variability and pathogenicity. Rhm51 is a single copy gene, and is constitutively expressed at low levels during the cell cycle and at higher levels when the pathogen is grown in media supplemented with DNA damaging agents. We disrupted Rhm51 in two different strains of M. oryzae and both mutants showed reduced growth with conidia and colonies being highly sensitive to DNA double strand break-inducing agents. In addition, they lost the ability to undergo homologous recombination in adeA gene targeting. Mutants showed reduction in conidiation compared to the wild-type strains. Although mutants infected compatible rice cultivars, they produced significantly smaller and fewer lesions compared to wild-type strains and this might be due to the reduction in the amount of appressoria formed. DNA double-strand breaks (DSBs) quantified by the comet assay showed that loss of Rhm51 resulted in the accumulation of DSBs in mutant. Microscopic observation of nuclei and reactive oxygen species (ROS) production during hyphal growth and following appressoria formation revealed impaired mitotic entry, autophagic cell death and ROS production in Rhm51 deletion mutants. Rhm51-GFP foci were observed in all stages of the asexual life cycle including the invasive hyphae formed in an intact rice leaf sheath, demonstrating that M. oryzae suffers DSBs during vegetative and infective growth.These results for the first time show that Rhm51 is involved in growth and pathogenicity of M. oryzae through the accurate repair of DSBs that may arise during vegetative and infective growth.

More books from GRIN Verlag

Cover of the book Die Problematik der Siegertribunale by Sali Atanga Ndindeng
Cover of the book Japan und Immigration by Sali Atanga Ndindeng
Cover of the book Yoga für den Rücken by Sali Atanga Ndindeng
Cover of the book Partnerwahl in der Türkei by Sali Atanga Ndindeng
Cover of the book Text-Analyse des Modus-Briefes von Nicolas Poussin by Sali Atanga Ndindeng
Cover of the book Wide-area measurement systems applied to voltage stability by Sali Atanga Ndindeng
Cover of the book Widerrufsrecht bei Verbraucherdarlehensverträgen. Eine Betrachtung zur Wirksamkeit der Anlage 2 zu § 14 Abs. 1 BGB-InfoV by Sali Atanga Ndindeng
Cover of the book Abweichendes Verhalten in der Schule by Sali Atanga Ndindeng
Cover of the book Implications of the Narrative Technique in Jane Austen's Emma by Sali Atanga Ndindeng
Cover of the book Auszeichnungen statt monetärer Entlohnung in Prinzipal-Agenten-Modellen by Sali Atanga Ndindeng
Cover of the book Sozialisationstheorie des Symbolischen Interaktionismus by Sali Atanga Ndindeng
Cover of the book Führung- und Führungskontexte mit Blick auf die stationäre Jugend- und Heimerziehung by Sali Atanga Ndindeng
Cover of the book Die Absatzplanung. Methoden, Instrumente und Prozeß by Sali Atanga Ndindeng
Cover of the book Japan - Land des Lächelns by Sali Atanga Ndindeng
Cover of the book Vom Phonograph zum CD-Player. Eine geschichtliche Aufbereitung für den Unterricht der ASO oder Integrationsklasse. by Sali Atanga Ndindeng
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy