A study on Aspergillus flavus

Biochemical characterization of Aspergillus flavus

Nonfiction, Health & Well Being, Medical, Medical Science, Microbiology
Cover of the book A study on Aspergillus flavus by Lisa Nathalie, GRIN Verlag
View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart
Author: Lisa Nathalie ISBN: 9783640989669
Publisher: GRIN Verlag Publication: August 22, 2011
Imprint: GRIN Verlag Language: English
Author: Lisa Nathalie
ISBN: 9783640989669
Publisher: GRIN Verlag
Publication: August 22, 2011
Imprint: GRIN Verlag
Language: English

Technical Report from the year 2011 in the subject Biology - Micro- and Molecular Biology, The University of Mysore (-), course: Biotechnology, language: English, abstract: Aspergillus flavus is the most widely known species of the genus Aspergillus which is known as a species in 1809 and first reported as a plant pathogen in 1920. Many fungi produce mycotoxin, a toxic secondary metabolite. Most A. flavus produces aflatoxin, a type of mycotoxin that is harmful for human, animal, and plant health. Characterization study of this saprophyte is important for the management of various human, animal, and plant diseases as well for surveillance, and other epidemiological study. The study focused on 3 subjects, morphological and physiological study, enzyme activity, and aflatoxin production from ground nut seeds collected from all over India. The growth of this fungus was compared in 4 different media for morphological study and in 4 different temperature on PDA media for physiological study. For enzyme activity, amylolytic, proteolytic, lipolytic, cellulolytic, pectinolytic and catalytic activity were determined. The presence of aflatoxin was performed using thin layer chromatography method. Among 12 isolates, amylase activity was shown by only 12 isolates and there is no clear zone in the remaining 8 isolates. Five isolates did not produce any protease among the 20 tested. Where as 18 isolates were positive for pectin methyl production. In case of lipase, 3 isolates did not show any white precipitation and all other isolates showed white precipitate due to the activity of lipase. Cellulose activity was very low in all the tested isolates and only 2 (AF7 and AF14) isolates exhibited the activity of cellulose in culture plates. Specific activity of catalase was calculated for all the isolates and all the isolates showed varied level of activity. The highest activity (210 mg/ml) was recorded by AF3 isolate and the least activity (1.58 mg/ml) in AF12. Among the 20 of isolates tested, 12 isolates of Aspergillus flavus were toxigenic by fluorescing blue spots for the presence of AFB1/AFB2 or both and 8 isolates. In summary, morphological, physiological, and biochemical studies are important to identify plant pathogenic fungus such Aspergillus flavus. Aflatoxin, being the widely produced mycotoxin of A. flavus is easily contaminates food and soil, thus its detection is required. Furthermore, by screening the extracellular enzymes activity, one can understand the ability of particular fungus to produce certain enzymes that have major contribution in biotechnology.

View on Amazon View on AbeBooks View on Kobo View on B.Depository View on eBay View on Walmart

Technical Report from the year 2011 in the subject Biology - Micro- and Molecular Biology, The University of Mysore (-), course: Biotechnology, language: English, abstract: Aspergillus flavus is the most widely known species of the genus Aspergillus which is known as a species in 1809 and first reported as a plant pathogen in 1920. Many fungi produce mycotoxin, a toxic secondary metabolite. Most A. flavus produces aflatoxin, a type of mycotoxin that is harmful for human, animal, and plant health. Characterization study of this saprophyte is important for the management of various human, animal, and plant diseases as well for surveillance, and other epidemiological study. The study focused on 3 subjects, morphological and physiological study, enzyme activity, and aflatoxin production from ground nut seeds collected from all over India. The growth of this fungus was compared in 4 different media for morphological study and in 4 different temperature on PDA media for physiological study. For enzyme activity, amylolytic, proteolytic, lipolytic, cellulolytic, pectinolytic and catalytic activity were determined. The presence of aflatoxin was performed using thin layer chromatography method. Among 12 isolates, amylase activity was shown by only 12 isolates and there is no clear zone in the remaining 8 isolates. Five isolates did not produce any protease among the 20 tested. Where as 18 isolates were positive for pectin methyl production. In case of lipase, 3 isolates did not show any white precipitation and all other isolates showed white precipitate due to the activity of lipase. Cellulose activity was very low in all the tested isolates and only 2 (AF7 and AF14) isolates exhibited the activity of cellulose in culture plates. Specific activity of catalase was calculated for all the isolates and all the isolates showed varied level of activity. The highest activity (210 mg/ml) was recorded by AF3 isolate and the least activity (1.58 mg/ml) in AF12. Among the 20 of isolates tested, 12 isolates of Aspergillus flavus were toxigenic by fluorescing blue spots for the presence of AFB1/AFB2 or both and 8 isolates. In summary, morphological, physiological, and biochemical studies are important to identify plant pathogenic fungus such Aspergillus flavus. Aflatoxin, being the widely produced mycotoxin of A. flavus is easily contaminates food and soil, thus its detection is required. Furthermore, by screening the extracellular enzymes activity, one can understand the ability of particular fungus to produce certain enzymes that have major contribution in biotechnology.

More books from GRIN Verlag

Cover of the book Quellenkritik: H. U. Abshagen: 'Generation ahnungslos - Momentaufnahmen eines 17-jährigen' by Lisa Nathalie
Cover of the book Der politische Liberalismus in der Revolution von 1848/49 - Geschichte und historische Bedeutung der 'Verfassung des Deutschen Reiches' by Lisa Nathalie
Cover of the book Legasthenie. Der Stellenwert der Sozialpädagogik im Konflikt zwischen Familie und Schule by Lisa Nathalie
Cover of the book Analyse des Lebensstandards eines Kindes von auf Hartz IV angewiesenen Eltern by Lisa Nathalie
Cover of the book Die Revolution 1848/49 - Deutsche Nationsbildung im Zeichen von Einheit und Freiheit by Lisa Nathalie
Cover of the book Japan und die Wirtschaftskrisen. Zeigt die globale Finanzkrise, dass die 'Lost Decade' eine lehrreiche Phase war, die Japan bei der Krisenbewältigung hilft? by Lisa Nathalie
Cover of the book Case study and comparative strategic analysis of Toyota and Ryanair by Lisa Nathalie
Cover of the book Die Auswirkungen von Anonymität in der Online-Kommunikation by Lisa Nathalie
Cover of the book Geschlecht und Führung. Kulturabhängige Aspekte in psychologischen Studien aus Japan by Lisa Nathalie
Cover of the book Aggressives Verhalten und Delinquenz by Lisa Nathalie
Cover of the book Evolutionäre Algorithmen in der Spracherkennung by Lisa Nathalie
Cover of the book Auswirkung von Botulinumtoxin Typ A Injektionen auf das Gangtraining bei zerebralparesebedingten spastischen Fußdeformitäten by Lisa Nathalie
Cover of the book Das Bedingungslose Grundeinkommen als Subvention von Niedriglöhnen by Lisa Nathalie
Cover of the book War Macht das unmittelbare Ziel für die Akteure der Berlinblockade 1948/49? by Lisa Nathalie
Cover of the book Difficulties of teaching English to native Bengali speakers by Lisa Nathalie
We use our own "cookies" and third party cookies to improve services and to see statistical information. By using this website, you agree to our Privacy Policy